Estudo comparativo entre as provas de imunodifusao em placa e em lamina na deteccao de anticorpos contra o virus da leucose enzootica bovina []. Contagem linfocitaria e anticorpos contra o virus da leucose enzootica bovina em rebanhos do Rio de Janeiro []. Ferreira, M.I. Romero, C.H. Rowe, C.A. Ocorrência do vírus da leucose enzoótica dos bovinos (BLV) e de anticorpos contra herpesvírus bovino tipo-1 (BoHV-1) e vírus da diarreia viral bovina (BVDV) .

Author: Zuktilar Tujind
Country: Congo
Language: English (Spanish)
Genre: Life
Published (Last): 26 October 2004
Pages: 341
PDF File Size: 7.78 Mb
ePub File Size: 5.16 Mb
ISBN: 755-2-72018-702-1
Downloads: 76871
Price: Free* [*Free Regsitration Required]
Uploader: Dojin

Once infected, the bovine remains as a virus carrier for life and such state correlates with the specific antibody detectable level. But neither leukemia nor other signs of infection could be detected. Transmission through procedures that transmit blood between animals such as gouge dehorning, vaccination and ear tagging with instruments or needles that are not changed or ezootica between animals is a significant means of BLV spread.

The variety of organs where white blood cells are to be found explains the many symptoms observed by veterinarians: Services on Demand Journal. Determination of the optimal cutoff value for a serological assay: CD5 is associated from the B-cell receptor in B cells from bovine leukemia virus-infected, persistently lymphocytotic cattle: Natural infection of animals other than cattle and buffalo are rare, although many animals are susceptible to artificial infection.

This correspondence is bovija That value showed the best correspondence between our challenge test Leucokit-La Plata and the gold standard test ID. Molecular diagnostic procedures PCR should not necessarily replace serological procedures, like ELISA, currently in use that had been proben to be cost-effective, rapid, sensitive and reliable and which are recognised as official test for commerce and trade purposes.

Enzootic bovine leukosis: performance of an indirect ELISA applied in serological diagnosis

Application in diagnosis of bovine leukaemia virus BLV infection in natutally infected cattle. Wochenschr5: Recebido em 19 de abril de Aceito em 13 de abril de Evaluation of alternative methods for the detection of bovine leukaemia virus in cattle.


A persistent antibody response can also be detected after experimental infection in deer, rabbits, rats, guinea-pigs, cats, dogs, sheep, rhesus monkeys, chimpanzees, antelopes, pigs, goats and buffaloes.

Possibilities and limitations for use of the polymerase chain reaction PCR in the diagnosis of bovine leukemia virus BLV infection in cattle. Only some animals later develop a B-cell leukemia called enzootic bovine leukosis. Reduced cell turnover in bovine leukemia virus-infected, persistently lymphocytotic cattle.

Leucose enzoótica bovina

Bovine Leukosis Virus EBLV is a widely distributed pathogen agent in the bovine population of many countries, especially in dairy cattle. Frontiers in Biosciencev. Benefits and limitations of polymerase chain reaction PCR in veterinary diagnostic virology.

It should be taken into account that the best “gold standard” test is a direct method or combination of methods that unequivocally identifies the virus carrier animal Veterinary BulletinVol. The cut-off value of a diagnostic test is the scale point of measure from which the quantitative results are classified as positive or negative and it is the value from which the quantitative data are categorised.

Sheep are very susceptible to experimental inoculation and lecuose tumours eznootica often and at a younger age than cattle. Ccomo descrito por Lyons e ParishLyons e Hawkins et al.

Sakai II ; K. The Kappa coefficient 14,18 measures the agreement level when two tests classify the results in two or more excluding categories infected, non infected.

There is, however, no conclusive evidence of transmission, and it is now generally thought that BLV is not a hazard to humans. On the other hand, the infected individuals are classified as true positives TP and as false negatives FN unless the test is perfectly sensitive where only TP results are obtained.


Values close to 0 denote discordance between the tests. The correspondence between both tests is De fato Debacq et al. These efforts are based on the application of tests such as immunodiffusion IDenzime linked immunosorbent assay ELISAwestern blot WB and polymerase chain reaction PCR ,13,15,20,24,25following the separation or removal of the virus-carrier animals.

Consultado em 17 de agosto de Some long term studies may be necessary, as there appears to be a correlation in instances of cancer among butchers and slaughterhouse workers. No treatment is available for the disease. Soon the United Kingdom followed.

Dtsch Tierarztl Wochenschr But it is not known whether this naturally occurring rabbit disease is linked to BLV infection. There are several methodologies to find that value depending enzooticw the type of values distribution 7,11, The PCR is a technique especially appropiated for the diagnosis of virus infection where virus specific antibodies cannot be detected and the presence of viral genome is the only evidence of infection Microbes and Infectionp.

Flow cytometry detection of phosphatidylserine expression on early apoptotic cells using fluorescein labelled Annexin-V. The predictive Leucokit-La Boviha values recommend its application in the commercialization of live cows export cow selection for hemo-vaccines and implementation of control and eradication programmes.

Productive and reproductive performance in cattle infected with bovine leukosis virus. The first country considered to be free of infection was Denmark.

Obtida de ” https: In these cases and as pointed out by Kozaczynska 15sample analyses should be repeated, keeping the animals as no conclusive until the definite confirmation enzootjca the result is obtained. Artigos sem imagem tanto localmente quanto no Wikidata!